Recombinant Alpaca anti-小鼠 IgG1 Antibody (Alexa Fluor 647)
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北京 101111
Quick Overview for Recombinant Alpaca anti-小鼠 IgG1 Antibody (Alexa Fluor 647) (ABIN7272920)
抗原
抗体类型
片段
克隆类型
标记
应用范围
克隆位点
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适用
- 小鼠
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宿主
- Alpaca
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Expression System
- E.coli
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原理
- Camelid sdAb anti-Mouse IgG1Alexa Fluor™ 647, Clone(s) 10A4
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特异性
- Mouse IgG1, does not cross-react with rabbit, guinea pig or chicken immunoglobulins
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纯化方法
- Produced in: E.coli
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免疫原
- This camelid sdAB anti-Mouse IgG1 is a species-specific antibody directed against IgG1 from Mouse. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications. Our anti-mouse IgG1 sdAbs bind specifically and strongly to mouse IgG1 independently of the IgGs light chains.
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Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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Goat anti-Mouse IgG1 antibody (PE) - Preadsorbed
FACS, FLISA Polyclonal IgG PE F(ab')2 fragment
Goat anti-Mouse IgG1 antibody - PreadsorbedELISA, WB, FACS, ICC, IHC (fro), IHC (p), ELISpot, MA, IHC (wm), IEM, SPR Polyclonal IgG unconjugated
Goat anti-Mouse IgG1 antibody (FITC) - PreadsorbedWB, FACS, ICC, FLISA, IHC (fro), IHC (p), IHC (wm) Polyclonal IgG FITC
Goat anti-Mouse IgG1 antibody (TRITC) - PreadsorbedICC, FLISA, IHC (fro), IHC (p), IHC (wm) Polyclonal IgG TRITC
Goat anti-Mouse IgG1 antibody (Cy5) - PreadsorbedWB, FACS, ICC, FLISA, IHC (fro), IHC (p), IHC (wm) Polyclonal IgG Cy5
Goat anti-Mouse IgG1 antibody (HRP) - PreadsorbedELISA, WB, ICC, IHC (fro), IHC (p), ELISpot, IHC (wm) Polyclonal IgG HRP
Goat anti-Mouse IgG1 antibody (Biotin) - PreadsorbedELISA, WB, FACS, ICC, IHC (fro), IHC (p), ELISpot, MA, IHC (wm), IEM Polyclonal IgG Biotin
Goat anti-Mouse IgG1 antibody (PE) - PreadsorbedFACS, FLISA, MA Polyclonal IgG PE
Rat anti-Mouse IgG1 antibody (HRP)ELISA, WB, ICC, IHC (fro), IHC (p) Monoclonal SB77e IgG2b HRP
Rat anti-Mouse IgG1 antibodyELISA, WB, FACS, ICC, IHC (fro), IHC (p) Monoclonal SB77e IgG2b unconjugated
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应用备注
- 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed)
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说明
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Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM
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缓冲液
- The single sdAb clone was lyophilized from PBS pH 7.4.
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储存条件
- -20 °C/-80 °C
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储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- IgG1
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物质类
- Antibody
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背景
- In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.
抗原
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