Recombinant Alpaca anti-小鼠 IgG Antibody (Atto 488)
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北京 101111
Quick Overview for Recombinant Alpaca anti-小鼠 IgG Antibody (Atto 488) (ABIN7272916)
抗原
抗体类型
片段
克隆类型
标记
应用范围
克隆位点
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适用
- 小鼠
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宿主
- Alpaca
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Expression System
- E.coli
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特异性
- Mouse IgG (Kappa light chain), does not cross-react with rabbit, guinea pig or chicken immunoglobulins
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纯化方法
- Produced in: E.coli
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免疫原
- Our sdAbs anti-mouse KLC specifically binds to all mouse Ig isotypes carrying a kappa light chain.
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Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM, DB Polyclonal IgG TRITC
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE F(ab')2 fragment
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应用备注
- IF: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM
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缓冲液
- The single sdAb clone was lyophilized from PBS pH 7.4.
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- IgG
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物质类
- Antibody
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背景
- In most research monoclonal antibodies are generated in mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be further categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to the subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.
抗原
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