Recombinant Alpaca anti-小鼠 IgG Antibody (Alexa Fluor 568)
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- 抗原 See all IgG products
- IgG
- 抗体类型
- Recombinant Antibody
- 片段
- single-domain Antibody (sdAb)
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适用
- 小鼠
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宿主
- Alpaca
- Expression System
- E.coli
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克隆类型
- 单克隆
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标记
- Alexa Fluor 568
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应用范围
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Mouse IgG (Kappa light chain), does not cross-react with rabbit, guinea pig or chicken immunoglobulins
- 纯化方法
- Produced in: E.coli
- 免疫原
- Our sdAbs anti-mouse KLC specifically binds to all mouse Ig isotypes carrying a kappa light chain.
- 克隆位点
- 1A23
- Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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Goat anti-Mouse IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
Goat anti-Mouse IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB, FACS, FLISA, FM, IP, LF Polyclonal IgG unconjugated
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- 应用备注
- IF: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM
- 缓冲液
- The single sdAb clone was lyophilized from PBS pH 7.4.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- In most research monoclonal antibodies are generated in mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be further categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to the subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.
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