Recombinant Alpaca anti-小鸡 IgY Antibody (Atto 488)
-
- 抗原 See all IgY products
- IgY
- 抗体类型
- Recombinant Antibody
- 片段
- single-domain Antibody (sdAb)
-
适用
- 小鸡
-
宿主
- Alpaca
- Expression System
- E.coli
-
克隆类型
- 单克隆
-
标记
- Atto 488
-
应用范围
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC)
- 特异性
- Chicken IgY, does not cross-react with mouse, rabbit, guinea pig or rat immunglobulins
- 纯化方法
- Produced in: E.coli
- 免疫原
- anti-Chicken is a species-specific FluoTag directed against immunoglobulins from Chicken.
- 克隆位点
- 3G2
- Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
-
Chicken anti-Chicken IgY antibody (DyLight 405)
IF, WB Polyclonal IgY DyLight 405
Chicken anti-Chicken IgY antibody (DyLight 488)IF, WB Polyclonal IgY DyLight 488
Chicken anti-Chicken IgY antibody (DyLight 633)IF, WB Polyclonal IgY DyLight 633
Chicken anti-Chicken IgY antibody (DyLight 550)IF, WB Polyclonal IgY DyLight 550
Goat anti-Chicken IgY (Heavy & Light Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Rabbit anti-Chicken IgY antibody (DyLight 488)IF Polyclonal IgG DyLight 488
Rabbit anti-Chicken IgY antibody (DyLight 549)IF Polyclonal IgG DyLight 549
-
- 应用备注
- IF: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM
- 缓冲液
- The single sdAb clone was lyophilized from PBS pH 7.4.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
-
- 抗原
- IgY
- Abstract
- IgY 产品
- 物质类
- Antibody
- 背景
- The immune repertoire of chickens is much simpler compared to the one of mammals, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.
-
