Recombinant Alpaca anti-小鸡 IgY Antibody (Alexa Fluor 647)
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Quick Overview for Recombinant Alpaca anti-小鸡 IgY Antibody (Alexa Fluor 647) (ABIN7272912)
抗原
抗体类型
片段
克隆类型
标记
应用范围
克隆位点
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适用
- 小鸡
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宿主
- Alpaca
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Expression System
- E.coli
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原理
- Camelid sdAb anti-Chicken IgY Alexa Fluor™ 647, Clone(s) 3G2
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特异性
- Chicken IgY, does not cross-react with mouse, rabbit, guinea pig or rat immunglobulins
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纯化方法
- Produced in: E.coli
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免疫原
- anti-Chicken is a species-specific camelid sdAB directed against immunoglobulins from Chicken.
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Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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Goat anti-Chicken IgY antibody (DyLight 488)
IF, WB Polyclonal IgY DyLight 488
Goat anti-Chicken IgY antibody (DyLight 633)IF, WB Polyclonal IgY DyLight 633
Goat anti-Chicken IgY antibody (DyLight 405)IF, WB Polyclonal IgY DyLight 405
Rabbit anti-Chicken IgY antibody (DyLight 488)IF Polyclonal IgG DyLight 488
Rabbit anti-Chicken IgY antibody (DyLight 549)IF Polyclonal IgG DyLight 549
Goat anti-Chicken IgY (Heavy & Light Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Chicken IgY antibody (DyLight 550)IF, WB Polyclonal IgY DyLight 550
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应用备注
- IF: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)
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说明
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Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM
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缓冲液
- The single sdAb clone was lyophilized from PBS pH 7.4.
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- IgY
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物质类
- Antibody
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背景
- The immune repertoire of chickens is much simpler compared to the one of mammals, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.
抗原
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