山羊 anti-小鼠 IgG (Light Chain) Antibody (HRP)
Quick Overview for 山羊 anti-小鼠 IgG (Light Chain) Antibody (HRP) (ABIN7205196)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Light Chain
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适用
- 小鼠
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宿主
- 山羊
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原理
- HRP, Goat Anti-Mouse IgG LCS
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特异性
- The antibody reacts with kappa light chains on mouse IgG, while it doesn't react with the heavy chain of mouse IgG. It's cross-reaction with human, rabbit, rat, goat, sheep and bovine serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins
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产品特性
- HRP conjugated Goat Anti-Mouse IgG light chain specific secondary antibody,Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.,Goat Anti-Mouse IgG
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纯化方法
- Affinity purified using solid phase Mouse IgG(H+L) light chain with finally > 95% purity based on SDS-PAGE
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免疫原
- Mouse IgG light chain
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:10000), IHC-P (1:50-1:500), ELISA (1:500-1:10000).
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说明
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50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination. This product solve these problems through less background noise, and enhanced accuracy. Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.4, containing 1 % BSA as stablizer and 50 % Glycerol.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- IgG
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物质类
- Antibody
抗原
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