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山羊 anti-大鼠 IgG Antibody (DyLight 680) - Preadsorbed

WB, FLISA, FM Polyclonal IgG DyLight 680

Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins). (山羊 anti-大鼠 IgG Antibody (DyLight 680) - Preadsorbed)

1 image

(1 reference)

产品编号 ABIN6699164

Supplier Product No.: 612-144-120

发货至: 中国

Datasheet as PDF

Quick Overview for 山羊 anti-大鼠 IgG Antibody (DyLight 680) - Preadsorbed (ABIN6699164)

抗原

IgG

克隆类型

多克隆

标记

DyLight 680

应用范围

Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

亚型

IgG

适用
752

703

607

467

410

244

213

176

146

141

124

110

107

101

82

62

47

28

27

16

15

10

8

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
大鼠
宿主
1772

1583

567

282

122

112

41

32

22

21

15

11

11
山羊

Supplier Product No.

612-144-120

Supplier

Rockland

原理

Rat IgG (H&L) Antibody DyLight™ 680 Conjugated Pre-Adsorbed

交叉反应（详细）

Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins

产品特性

Goat Anti-Rat IgG DyLight 680™ Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 680™ Conjugation,Anti-Rat IgG (H&L) DyLight™680 Antibody generated in goat detects reactivity to Rat IgG.

纯化方法

Preadsorption: Pre-Adsorbed

免疫原

Rat IgG whole molecule

Labeling Ratio

2.1
Goat anti-Rat IgG (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

Rabbit anti-Mouse IgG antibody (HRP) - Preadsorbed
ELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment

Goat anti-Rat IgG antibody (DyLight 488)
WB, FLISA, FM Polyclonal IgG DyLight 488

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG Atto 647N

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 550

Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Rat IgG antibody (Cy3) - Preadsorbed
WB, FACS, FLISA, FM, DB Polyclonal IgG Cy3

Goat anti-Rat IgG (Heavy Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

Goat anti-Rat IgG (Heavy Chain) antibody (beta-Gal)
ELISA, IHC, WB Polyclonal IgG beta-Gal

应用备注

FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized

说明

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: WB

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Cartagena-Rivera, Van Itallie, Anderson, Chadwick: "Apical surface supracellular mechanical properties in polarized epithelium using noninvasive acoustic force spectroscopy." in: Nature communications, Vol. 8, Issue 1, pp. 1030, (2018) (PubMed).
抗原

IgG

物质类

Antibody

背景

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG is conjugated to DyLight™680.

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