山羊 anti-兔 IgG Antibody (Cy2) - Preadsorbed
(3 references)
Quick Overview for 山羊 anti-兔 IgG Antibody (Cy2) - Preadsorbed (ABIN6699089)
抗原
克隆类型
标记
应用范围
亚型
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适用
- 兔
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宿主
- 山羊
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Supplier Product No.
- 611-111-122
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Supplier
- Rockland
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原理
- Rabbit IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins
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产品特性
- Goat Anti Rabbit IgG Antibody CY2 Conjugation, Goat Anti-Rabbit IgG CY2 Conjugated Antibody,Anti-Rabbit IgG (H&L) Antibody CY2 Conjugated generated in goat detects reactivity to Rabbit IgG.
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纯化方法
- Preadsorption: Pre-Adsorbed
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免疫原
- Rabbit IgG whole molecule
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Labeling Ratio
- 4.5
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Guinea Pig anti-Rabbit IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, CUT&Tag, CUT&RUN Polyclonal IgG unconjugated
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC)WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)High-quality product from Rockland ELISA, IHC, WB, DB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 800)ELISA, WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Mouse anti-Rabbit IgG antibody (HRP)Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 488)WB, IF, FLISA, FM, DB, MA Polyclonal IgG DyLight 488
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应用备注
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FLISA_Dilution: 1:10,000 - 1:50,000
ELISA_Dilution: 1:10,000 - 1:50,000
Flow_Cytometry_Dilution: 1:500 - 1:2,500
IF_Microscopy_Dilution: 1:1,000 - 1:5,000
Western_Blot_Dilution: User Optimized
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说明
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Anti-Rabbit IgG (H&L) Conjugated Cy2 Secondary Antibody has been tested by dot blot and western bot. This antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: IF, Multiplex -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "SDF1 gradient associates with the distribution of c-Kit+ cardiac cells in the heart." in: Scientific reports, Vol. 8, Issue 1, pp. 1160, (2018) (PubMed).
: "Nicotinamide adenine dinucleotide-dependent binding of the neuronal Ca2+ sensor protein GCAP2 to photoreceptor synaptic ribbons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 19, pp. 6559-76, (2010) (PubMed).
: "CXCR4 modulates contractility in adult cardiac myocytes." in: Journal of molecular and cellular cardiology, Vol. 41, Issue 5, pp. 834-44, (2007) (PubMed).
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: "SDF1 gradient associates with the distribution of c-Kit+ cardiac cells in the heart." in: Scientific reports, Vol. 8, Issue 1, pp. 1160, (2018) (PubMed).
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- IgG
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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