兔 anti-小鼠 IgG2b (Heavy Chain) Antibody (DyLight 488)
Quick Overview for 兔 anti-小鼠 IgG2b (Heavy Chain) Antibody (DyLight 488) (ABIN6699070)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy Chain
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适用
- 小鼠
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宿主
- 兔
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Supplier Product No.
- 610-441-042
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Supplier
- Rockland
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原理
- Mouse IgG2b (Gamma 2b chain) Antibody DyLight™ 488 Conjugated
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产品特性
- Rabbit Anti-Mouse IgG2b (Gamma 2b chain) Antibody DyLight 488™ Conjugated, Rabbit Anti Mouse IgG2b DyLight 488™ Conjugated Antibody,Anti-Mouse IgG2b DyLight 488 Antibody generated in rabbit detects reactivity to Mouse IgG2b (Gamma 2b chain).
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免疫原
- Mouse IgG2b heavy chain
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Labeling Ratio
- 3.1
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Rabbit anti-Mouse IgG2b antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgG2b (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Rat anti-Mouse IgG2b antibody (FITC)ELISA, FACS, FLISA Monoclonal SB74g IgG2b FITC
Rat anti-Mouse IgG2b antibody (PE)ELISA, FACS, FLISA Monoclonal SB74g IgG2b PE
Goat anti-Mouse IgG2b antibody (Alkaline Phosphatase (AP))ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgG2b antibodyELISA, FLISA Polyclonal IgG unconjugated
Goat anti-Mouse IgG2b (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rat anti-Mouse IgG2b antibodyELISA, FACS, FLISA Monoclonal SB74g IgG2b unconjugated
Goat anti-Mouse IgG2b antibody (Biotin)ELISA, FLISA Polyclonal IgG Biotin
Rat anti-Mouse IgG2b antibody (HRP)ELISA, FACS, FLISA Monoclonal SB74g IgG2b HRP
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应用备注
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized
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说明
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This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG2b
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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