兔 anti-小鼠 IgG (Fc Region) Antibody (DyLight 800) - Preadsorbed
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Quick Overview for 兔 anti-小鼠 IgG (Fc Region) Antibody (DyLight 800) - Preadsorbed (ABIN6699051)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Fc Region
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适用
- 小鼠
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宿主
- 兔
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Supplier Product No.
- 610-445-003
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Supplier
- Rockland
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产品特性
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Synonyms: Rabbit Anti-Mouse IgG F(c) Antibody DyLight™ 800 Conjugation, Rabbit Anti-Mouse IgG Fc Antibody DyLight™ 800 Conjugated, Rabbit Anti-Mouse IgG Fc Fragment DyLight™800 Conjugated Antibody
Background: Anti-Mouse IgG F(c) DyLight antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
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纯化方法
- Preadsorption: immunoaffinity chromatography using Mouse IgG coupled to agarose beads
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免疫原
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Immunogen: Anti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in rabbit.
Immunogen Type: Native Protein
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Labeling Ratio
- 1.9
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM, DB Polyclonal IgG TRITC
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (APC) - PreadsorbedELISA, FACS Polyclonal IgG APC
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应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Mouse IgG F(c) DyLight™800 Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
ELISA Dilution: 1:40,000 - 1:180,000
Western Blot Dilution: 1:2,000 - 1:10,000
IF Microscopy Dilution: >1:5,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- RT,4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
抗原
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