(1 reference)
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- 抗原 See all IgG products
- IgG
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适用
- 小鼠
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宿主
- 驴
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克隆类型
- 多克隆
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标记
- Cy5.5
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应用范围
- FLISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 610-713-124
- Supplier
- Rockland
- 原理
- Mouse IgG (H&L) Antibody CY5.5 Conjugated Pre-Adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins
- 产品特性
- Donkey Anti-Mouse IgG (H&L) Antibody CY5.5 Conjugated Pre-Adsorbed, Donkey Anti Mouse IgG Antibody CY5.5 Conjugated,Anti-Mouse IgG Cy5.5 Antibody generated in donkey detects reactivity to Mouse IgG.
- 纯化方法
- Preadsorption: Pre-Adsorbed
- 免疫原
- Mouse IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 7.6
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM, DB Polyclonal IgG TRITC
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Rabbit anti-Mouse IgG antibody (Atto 550)WB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Mouse IgG antibody (DyLight 549) - PreadsorbedELISA, WB, FLISA, FM, DB Polyclonal IgG DyLight 549
Donkey anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Atto 425) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG Atto 425
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Atto 594) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 594
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- 应用备注
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FLISA_Dilution: 1:10,000 - 1:50,000
IF_Microscopy_Dilution: 1:1,000 - 1:5,000
Other: User Optimized
- 说明
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This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Mammalian late vacuole protein sorting orthologues participate in early endosomal fusion and interact with the cytoskeleton." in: Molecular biology of the cell, Vol. 15, Issue 3, pp. 1197-210, (2004) (PubMed).
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: "Mammalian late vacuole protein sorting orthologues participate in early endosomal fusion and interact with the cytoskeleton." in: Molecular biology of the cell, Vol. 15, Issue 3, pp. 1197-210, (2004) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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