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山羊 anti-小鼠 IgG Antibody (Cy3) - Preadsorbed

Rockland 610-104-121 WB, FACS, FLISA, FM Polyclonal IgG Cy3
Rockland
产品编号 ABIN6698986
Supplier Product No.: 610-104-121
发货至: 中国
  • 抗原 See all IgG products
    IgG
    适用
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    小鼠
    宿主
    • 1766
    • 1547
    • 569
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    山羊
    克隆类型
    • 3395
    • 175
    • 4
    • 1
    多克隆
    标记
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    Cy3
    应用范围
    • 2044
    • 1987
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    Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Supplier Product No.
    610-104-121
    Supplier
    Rockland
    原理
    Mouse IgG (H&L) Antibody CY3 Conjugated Pre-Adsorbed
    交叉反应 (详细)
    Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins
    产品特性
    Goat Anti-Mouse IgG (H&L) Antibody CY3 Conjugated Pre-Adsorbed, Goat Anti Mouse IgG Antibody CY3 Conjugated,Anti-Mouse IgG Cy3 Antibody generated in goat detects reactivity to Mouse IgG.
    纯化方法
    Preadsorption: Pre-Adsorbed
    免疫原
    Mouse IgG whole molecule
    亚型
    IgG
    Labeling Ratio
    8.5
  • 应用备注

    FLISA_Dilution: 1:10,000 - 1:50,000

    Flow_Cytometry_Dilution: 1:500 - 1:2,500

    IF_Microscopy_Dilution: 1:1,000 - 1:5,000

    Other: FLOW CYTOMETRY 1:500 - 1:2,500

    说明

    Anti-Mouse IgG Cy3 Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Suggested Applications: IF, IHC, Multiplex

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Ng-Blichfeldt, Stewart, Clatworthy, Williams, Röper: "Identification of a core transcriptional program driving the human renal mesenchymal-to-epithelial transition." in: Developmental cell, Vol. 59, Issue 5, pp. 595-612.e8, (2024) (PubMed).

    Hartmann, Raabe, Wenisch, Arnhold: "Amniotic fluid derived stem cells give rise to neuron-like cells without a further differentiation potential into retina-like cells." in: American journal of stem cells, Vol. 2, Issue 2, pp. 108-18, (2013) (PubMed).

    Venkatesan, Natarajan, Schwarz, Mayer, Alpadi, Magupalli, Sung, Schmitz: "Nicotinamide adenine dinucleotide-dependent binding of the neuronal Ca2+ sensor protein GCAP2 to photoreceptor synaptic ribbons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 19, pp. 6559-76, (2010) (PubMed).

    Arnhold, Post, Glüer, Hoopmann, Wenisch, Volpers, Addicks: "Neuronal characteristics of amniotic fluid derived cells after adenoviral transformation." in: Cell biology international, Vol. 32, Issue 12, pp. 1559-66, (2009) (PubMed).

    Pyo, Sui, Dhume, Palomeque, Blaxall, Diaz, Tunstead, Logothetis, Hajjar, Schecter: "CXCR4 modulates contractility in adult cardiac myocytes." in: Journal of molecular and cellular cardiology, Vol. 41, Issue 5, pp. 834-44, (2007) (PubMed).

    Arnhold, Klein, Klinz, Absenger, Schmidt, Schinköthe, Brixius, Kozlowski, Desai, Bloch, Addicks: "Human bone marrow stroma cells display certain neural characteristics and integrate in the subventricular compartment after injection into the liquor system." in: European journal of cell biology, Vol. 85, Issue 6, pp. 551-65, (2007) (PubMed).

    Wolter, Tauer, Fuchs, Volk: "Mapping of cytoskeletal components in the hippocampal formation of the tree shrew (Tupaia belangeri)." in: Journal of chemical neuroanatomy, Vol. 17, Issue 2, pp. 65-74, (1999) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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