兔 anti-猴 IgG Antibody (DyLight 488) - Preadsorbed
Quick Overview for 兔 anti-猴 IgG Antibody (DyLight 488) - Preadsorbed (ABIN6698967)
抗原
克隆类型
标记
应用范围
亚型
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适用
- 猴
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宿主
- 兔
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Supplier Product No.
- 617-441-002
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Supplier
- Rockland
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产品特性
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Synonyms: rabbit anti-Monkey IgG DyLight™ 488 conjugated antibody, rabbit anti-Monkey IgG antibody DyLight™ 488 conjugation
Background: Conjugated Anti-Monkey IgG (H&L) DyLight™ 488 antibody generated in rabbit detects specifically monkey IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This DyLight™488 conjugated anti-Monkey IgG (H&L) secondary antibody is ideal for investigators who routinely perform immunofluorescence, flow cytometry, and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
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纯化方法
- Preadsorption: immunoaffinity chromatography using Monkey IgG coupled to agarose beads
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免疫原
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Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.
Immunogen Type: Native Protein
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Labeling Ratio
- 4.8
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Goat anti-Monkey IgG antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
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应用备注
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Application Note: Anti-Monkey IgG (H&L) DyLight™ 488 conjugated Antibody is suitable for use in immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity and signal detection should be optimized by the end user.
FLISA Dilution: >1:10,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- RT,4 °C,-20 °C
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储存方法
- Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
抗原
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