山羊 anti-猴 IgG (Chain gamma) Antibody (DyLight 488) - Preadsorbed
Quick Overview for 山羊 anti-猴 IgG (Chain gamma) Antibody (DyLight 488) - Preadsorbed (ABIN6698964)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Chain gamma
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适用
- 猴
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宿主
- 山羊
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Supplier Product No.
- 617-141-012
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Supplier
- Rockland
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产品特性
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Synonyms: goat anti-Monkey IgG (gamma chain) DyLight™488 conjugated antibody, goat anti-Monkey IgG gamma antibody DyLight™ 488 conjugation
Background: Anti-Monkey IgG (gamma chain) DyLight™488 conjugated antibody generated in goat detects specifically monkey IgG (gamma chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. This DyLight™488 conjugated anti-Monkey IgG gamma chain secondary antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.
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纯化方法
- Preadsorption: Solid phase absorption
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免疫原
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Immunogen: Anti-Monkey IgG (gamma chain) was produced by repeated immunization with monkey IgG gamma heavy chain in goat.
Immunogen Type: Native Protein
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Labeling Ratio
- 5.7
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Goat anti-Monkey IgG antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
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应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Monkey IgG (gamma chain) DyLight™488 is suitable for use in immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity and signal detection should be optimized by the end user.
FLISA Dilution: >1:20,000
Western Blot Dilution: 1:2,000 - 1:10,000
IF Microscopy Dilution: >1:5,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
抗原
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