驴 anti-人 IgG Antibody (Cy2) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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适用
- 人
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宿主
- 驴
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克隆类型
- 多克隆
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标记
- Cy2
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应用范围
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 609-711-123
- Supplier
- Rockland
- 原理
- Human IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed
- 交叉反应 (详细)
- Minimal crossreactivity against Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins
- 产品特性
- Donkey Anti Human IgG CY2 Conjugated Antibody, Donkey Anti-Human IgG Antibody CY2 Antibody,Anti-Human IgG (H&L) Cy2 generated in donkey detects human Immunoglobulin G (heavy and light chains).
- 纯化方法
- Preadsorption: Pre-Adsorbed
- 免疫原
- Human IgG whole molecule
- 亚型
- IgG
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- 应用备注
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FLISA_Dilution: 1:10,000 - 1:50,000
ELISA_Dilution: 1:10,000 - 1:50,000
Flow_Cytometry_Dilution: 1:500 - 1:2,500
IF_Microscopy_Dilution: 1:1,000 - 1:5,000
Western_Blot_Dilution: 1:1,000 - 1:5,000
- 说明
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Cy2 Conjugated Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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