兔 anti-绵羊 IgG Antibody (Cy5)
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- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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适用
- 绵羊
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Cy5
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应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 713-410-002
- Supplier
- Rockland
- 原理
- F(ab')2 Sheep IgG (H&L) Antibody Cy5 Conjugated
- 产品特性
- Rabbit F(ab')2 Anti-Sheep IgG CY5 Conjugated Antibody, Rabbit Fab2 Anti-Sheep IgG Antibody CY5 Conjugation,F(ab')2 Anti-Sheep IgG (H&L) Antibody generated in rabbit detects sheep IgG. Representing approximately 75 % of serum immunoglobulins, F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Sheep IgG Antibody is conjugated to CY5.
- 免疫原
- Sheep IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 21
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Donkey anti-Sheep IgG antibody (DyLight 680) - Preadsorbed
WB, FLISA, FM Polyclonal IgG DyLight 680
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- 应用备注
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FLISA_Dilution: 1:10,000 - 1:50,000
Flow_Cytometry_Dilution: 1:500 - 1:2,500
IF_Microscopy_Dilution: 1:1,000 - 1:5,000
Other: FLOW CYTOMETRY 1:500 - 1:2,500
- 说明
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This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 250 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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