兔 anti-人 IgM Heavy Chain Antibody
IHC (p)
Monoclonal
IGHM-2559R
IgG kappa
unconjugated
Quick Overview for 兔 anti-人 IgM Heavy Chain Antibody (ABIN6654259)
抗原
IgM Heavy Chain
克隆类型
单克隆
应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
IGHM-2559R
亚型
IgG kappa
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适用
- 人
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宿主
- 兔
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纯化方法
- Purified
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纯度
- Protein A affinity chromatography
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免疫原
- Recombinant heavy chain of human IgM was used as the immunogen for this recombinant IgM antibody.
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Mouse anti-Human IgM Heavy Chain antibody
IHC (p), WB, FACS, IF Monoclonal IM373 IgG1 kappa unconjugated
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应用备注
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The optimal dilution of the recombinant IgM antibody for each application should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) -
限制
- 仅限研究用
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- IgM Heavy Chain
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背景
- Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
抗原
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