山羊 anti-人 IgM (Chain mu) Antibody (HRP)
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北京 101111
Quick Overview for 山羊 anti-人 IgM (Chain mu) Antibody (HRP) (ABIN619752)
抗原
片段
克隆类型
标记
应用范围
亚型
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抗原表位
- Chain mu
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适用
- 人
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宿主
- 山羊
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交叉反应
- 人
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产品特性
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Goat anti-human IgM, µ chain, F(ab) '2 fragment is a HRP conjugated secondary antibody which is reacting with human IgM, µ chain, F(ab) '2. Antibody solution may contain small amounts of intact IgG.
F(ab)'2 fragment -
纯化方法
- Affinity purification
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纯度
- > 90 % based on SDS-PAGE.
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应用备注
- 1:500-1:5000 for immunohistochemistry (IHC), 1:10 000-1:50 000 for ELISA/western blot.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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注意事项
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Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. -
储存条件
- 4 °C
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储存方法
- Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- IgM
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物质类
- Antibody
抗原
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