山羊 anti-人 IgG (Fc Region) Antibody (Alkaline Phosphatase (AP))
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- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- Fc Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)
- 交叉反应
- 绵羊, 小鼠, 兔
- 预测反应
- human IgG Fc (two heavy chains with constant domains)
- 产品特性
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Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab) '2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
F(ab)'2 fragment - 纯化方法
- Affinity purification
- 免疫原
- Purified human IgG
- 亚型
- IgG
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- 应用备注
- 1: 500 - 2 000 (ELISA) and (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)
- 说明
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This antibody reacts with the heavy (gamma) chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins, or non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins or mouse and rabbit IgG based on immunoelectrophoresis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. - 储存条件
- 4 °C
- 储存方法
- Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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