山羊 anti-人 IgE (Chain epsilon) Antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC, ICC
Polyclonal
IgG
Alkaline Phosphatase (AP)
Quick Overview for 山羊 anti-人 IgE (Chain epsilon) Antibody (Alkaline Phosphatase (AP)) (ABIN619576)
抗原
IgE
克隆类型
多克隆
标记
Alkaline Phosphatase (AP)
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
亚型
IgG
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抗原表位
- Chain epsilon
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适用
- 人
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宿主
- 山羊
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交叉反应
- 绵羊, 小鼠, 兔
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预测反应
- human IgE heavy (epsilon chain)
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产品特性
- Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgE heavy (epsilon chain) in immunological assays.
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纯化方法
- Affinity purification
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免疫原
- purified human IgE
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Mouse anti-Human IgE (Fc Region) antibody (Biotin)
ELISA Monoclonal HP6029 IgG2a Biotin
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应用备注
- 1: 500 - 2 000 (ELISA) and (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)
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说明
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No reactivity is observed to light chains or non-IgE human serum proteins based in immunoelectrophoresisno reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis
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限制
- 仅限研究用
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状态
- Liquid
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注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. -
储存条件
- 4 °C
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储存方法
- Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- IgE
抗原
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