绵羊 anti-兔 IgG (Heavy & Light Chain) Antibody (TRITC)
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Quick Overview for 绵羊 anti-兔 IgG (Heavy & Light Chain) Antibody (TRITC) (ABIN619446)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 兔
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宿主
- 绵羊
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原理
- Sheep anti-Rabbit IgG (H&L), TRITC conjugated
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纯化方法
- Immunogen affinity purified
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免疫原
- Purified rabbit IgG (H&L)
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应用备注
- The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 1,1 mL of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
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缓冲液
- Immunogen affinity purified sheep IgG.
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注意事项
- Prepare working dilution prior to use and discard after use. Do not store working dilution,Be sure to mix well but without foaming
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储存条件
- 4 °C,-20 °C
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储存方法
- Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
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- IgG
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物质类
- Antibody
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背景
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Background: Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm, Emax = 570 nm.
Additional information: Purity of this preparation is > 95 % based on SDS-PAGE. Antibody concentration is 1.0 mg/mL (E 1 % at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins.
抗原
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