兔 anti-人 IgG (Heavy & Light Chain) Antibody (HRP)
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北京 101111
Quick Overview for 兔 anti-人 IgG (Heavy & Light Chain) Antibody (HRP) (ABIN619410)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 人
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宿主
- 兔
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产品特性
- Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to HRP, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
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纯化方法
- Affinity purification
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免疫原
- Purified human IgG (H&L) CAA10835
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应用备注
- This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:5 000 for Immunohistochemistry 1:200-1:5000 for ELISA/Western blot.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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注意事项
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Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. -
储存条件
- 4 °C
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储存方法
- Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- IgG
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物质类
- Antibody
抗原
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