兔 anti-人 IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))
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Quick Overview for 兔 anti-人 IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN619407)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 人
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宿主
- 兔
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原理
- Rabbit anti-Human IgG (H&L), ALP conjugated
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纯化方法
- Immunogen affinity purified
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免疫原
- Purified human IgG (H&L) CAA10835
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Mouse anti-Human IgG (Fc Region) antibody
Animal-Free ELISA, FACS, WB, IF Multiclonal IgG2a Recombinant Antibody unconjugated
Mouse anti-Human IgG (Fc Region) antibody (HRP)Animal-Free ELISA, FACS, WB, IF Multiclonal IgG2a Recombinant Antibody HRP
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Mouse anti-Human IgG (Fc Region) antibody (Alexa Fluor 555)Animal-Free ELISA, FACS, WB, IF Multiclonal IgG2a Recombinant Antibody Alexa Fluor 555
Mouse anti-Human IgG (Fc Region) antibody (Alexa Fluor 488)Animal-Free ELISA, FACS, WB, IF Multiclonal IgG2a Recombinant Antibody Alexa Fluor 488
Goat anti-Human IgG (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Human IgG (Heavy Chain) antibody (beta-Gal)ELISA, WB Polyclonal IgG beta-Gal
Goat anti-Human IgG (F(ab')2 Region) antibody - PreadsorbedELISA, IHC, WB, DB Polyclonal IgG unconjugated F(ab')2 fragment
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应用备注
- This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Immunogen affinity purified rabbit IgG.
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注意事项
- Prepare working dilution prior to use and discard after use. Do not store working dilution,Be sure to mix well but without foaming
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储存条件
- 4 °C,-20 °C
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储存方法
- Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
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有效期
- 48 months
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- IgG
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物质类
- Antibody
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背景
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Background: Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to ALP, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
Additional information: Purity of this preparation is > 95 % based on SDS-PAGE. Antibody concentration is 1.50 mg/mL (E 1 % at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins
抗原
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