Rabbit anti-chicken IgG/Y (H&L) is a secondary antibody conjugated to TRITC, which binds to chicken IgG/Y (H&L) in immunological assays. Antibody is affinity purified using solid phase chicken IgG/Y. Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm, Emax = 570 nm.
This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2000 for most applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
注意事项
Prepare working dilution prior to use and then discard. Product is photosensitive and should be protected from light.
储存条件
4 °C
储存方法
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.