山羊 anti-人 IgM (Chain mu) Antibody (Alkaline Phosphatase (AP))
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北京 101111
Quick Overview for 山羊 anti-人 IgM (Chain mu) Antibody (Alkaline Phosphatase (AP)) (ABIN619228)
抗原
片段
克隆类型
标记
应用范围
亚型
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抗原表位
- Chain mu
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适用
- 人
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宿主
- 山羊
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交叉反应
- 人
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产品特性
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Goat anti-human IgM (µ chain), ALP (alkaline phosphatase) conjugated, is a F(ab) '2 fragment of a secondary antibody which binds to human IgM in immunological assays. Antibodies are adsorbed against human IgG or IgA. Antibody solution may contain small amounts of intact IgG.
F(ab)'2 fragment -
纯化方法
- Affinity purification
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纯度
- > 90 % based on SDS-PAGE.
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应用备注
- 1:500-1:2000 for ELISA/western blot, 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC) and flow cytometry.
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限制
- 仅限研究用
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状态
- Liquid
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注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. -
储存条件
- 4 °C
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储存方法
- Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- IgM
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物质类
- Antibody
抗原
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