小鼠 anti-人 kappa Light Chain Antibody (CF®405S)
Quick Overview for 小鼠 anti-人 kappa Light Chain Antibody (CF®405S) (ABIN6173956)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
-
-
适用
- 人
-
宿主
- 小鼠
-
原理
- Mouse Monoclonal anti-Human Kappa Light Chain (HP6053), CF405S Conjugate
-
产品特性
- This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
免疫原
- Purified human Ig kappa chain
-
-
Goat anti-Human kappa Light Chain antibody (Biotin)
WB, ELISA, IHC Polyclonal IgG Biotin
Goat anti-Human kappa Light Chain antibody (FITC)FACS, WB, ELISA, FM, DB, FLISA Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Human kappa Light Chain antibody (Alkaline Phosphatase (AP))WB, ELISA, IHC Polyclonal IgG Alkaline Phosphatase (AP) F(ab')2 fragment
-
-
应用备注
-
Immunohistology formalin-fixed 0.5-1.0 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Western blotting 0.5-1.0 μg/mL
- Flow Cytometry 0.5-2.0 μg/million cells in 0.1 mL
- Optimal dilution for a specific application should be determined by user
-
说明
-
293T, Raji or hPBL cells. Tonsil or Spleen
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 100 μg/mL
-
缓冲液
- PBS/0.1 % BSA/0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Protect from light
-
-
- kappa Light Chain
-
背景
- HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM
-
分子量
- ~22.5 kDa
-
基因ID
- 3514, 449609
抗原
-
