小鼠 anti-人 IgA Antibody (CF®543)
Quick Overview for 小鼠 anti-人 IgA Antibody (CF®543) (ABIN6156271)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
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适用
- 人
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宿主
- 小鼠
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原理
- Mouse Monoclonal anti-Human IgA Immunoglobulin Immunoglobulin (IA761), CF543 Conjugate
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产品特性
- This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Alpha heavy chain. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins.IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkin s lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Purified human alpha heavy chain
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Goat anti-Human IgA antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgA antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human IgA (Heavy Chain) antibody (Biotin)ELISA, WB, FACS Polyclonal IgG Biotin
Goat anti-Human IgA (Heavy Chain) antibody (FITC)ELISA, FACS Polyclonal IgG FITC
Goat anti-Human IgA (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
Goat anti-Human IgA antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Human IgA antibody (FITC)ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Human IgA (Heavy Chain) antibody (Texas Red (TR))ELISA, FACS Polyclonal IgG Texas Red (TR)
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应用备注
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Immunohistology formalin-fixed 0.5-1.0 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1.0 μg/mL
- Flow Cytometry 1-2 μg/million cells in 0.1 mL
- Optimal dilution for a specific application should be determined by user
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说明
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Daudi, 293T, Raji or hPBL cells. Tonsil or Spleen.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- IgA
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别名
- IgA Immunoglobulin
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物质类
- Antibody
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背景
- A2m Marker, Ig alpha 1 Chain C Region, Ig alpha 2 Chain C Region, IGHA1, IGHA2, Immunoglobulin Am1, Immunoglobulin Am2, Immunoglobulin Heavy Constant Alpha 1, Immunoglobulin Heavy Constant Alpha 2
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分子量
- 50-75 kDa
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基因ID
- 3493, 3494, 699841
抗原
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