小鼠 anti-人 IgA Secretory Component Antibody
FACS, IF, IHC (f)
Monoclonal
ECM1-792
IgG1 kappa
unconjugated
1 image
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- 抗原
- IgA Secretory Component
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适用
- 人, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-IgA Secretory Component (ECM1/792), Purified, BSA-free
- 产品特性
- This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Recombinant human ECM1 protein
- 克隆位点
- ECM1-792
- 亚型
- IgG1 kappa
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- 应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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Stomach, Lung, or breast tumor
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (no BSA, no azide)
- 储存液
- Azide free
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- 抗原
- IgA Secretory Component
- 背景
- ECM1, Extracellular Matrix Protein 1, Secretory Component p85, URBWD
- 分子量
- ~80 kDa
- 基因ID
- 1893, 81071
- UniProt
- Q16610
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