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小鼠 anti-人 IgM Antibody (CF®405S)

FACS, IF, IHC (f) Monoclonal DA4-4 IgG1 kappa CF®405S
产品编号 ABIN6154935
发货至: 中国

Quick Overview for 小鼠 anti-人 IgM Antibody (CF®405S) (ABIN6154935)

抗原

IgM

克隆类型

  • 518
  • 108
单克隆

标记

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CF®405S

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

DA4-4

亚型

IgG1 kappa
  • 适用

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    宿主

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    小鼠

    原理

    Mouse Monoclonal anti-Human IgM Immunoglobulin (DA4-4), CF405S Conjugate

    产品特性

    This antibody recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Heavy chain of human IgM
  • 应用备注

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    说明

    293T, Raji or hPBL cells. Tonsil or Spleen

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    IgM

    别名

    IgM Immunoglobulin

    物质类

    Antibody

    背景

    AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region

    分子量

    50-75 kDa

    基因ID

    3507, 510635
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