兔 anti-猴 IgG (Heavy & Light Chain) Antibody (HRP)
ELISA, IHC, WB, IP
Polyclonal
IgG
HRP
1 image
Quick Overview for 兔 anti-猴 IgG (Heavy & Light Chain) Antibody (HRP) (ABIN5706888)
抗原
IgG
克隆类型
多克隆
标记
HRP
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)
亚型
IgG
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抗原表位
- Heavy & Light Chain
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适用
- 猴
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宿主
- 兔
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Supplier Product No.
- 617-403-002
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Supplier
- Rockland
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原理
- Monkey IgG (H&L) Antibody Peroxidase
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Peroxidase, Monkey IgG and Monkey Serum.
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产品特性
- Anti-Monkey IgG (H&L) FITC antibody generated in rabbit detects specifically monkey IgG heavy and light chains.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads.
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免疫原
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Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.
Immunogen Type: Native Protein
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Goat anti-Monkey IgG antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
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应用备注
- Application Note: Anti-Monkey IgG (H&L) HRP conjugated Antibody has been tested in ELISA and western blot and is suitable for use in Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:10,000 - 1:60,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: 1:50,000 - 1:100,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This peroxidase conjugated anti-Monkey IgG (H&L) secondary antibody is ideal for investigators who routinely perform western-blot, immunoprecipitation and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.
抗原
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