山羊 anti-人 IgA (Free & Bound), (Secretory Component) Antibody (FITC)
IF, IHC (fro), IP, EIA, ID
Polyclonal
IgG
FITC
Quick Overview for 山羊 anti-人 IgA (Free & Bound), (Secretory Component) Antibody (FITC) (ABIN568606)
抗原
IgA
克隆类型
多克隆
标记
FITC
应用范围
Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID)
亚型
IgG
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抗原表位
- Free & Bound, Secretory Component
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适用
- 人
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宿主
- 山羊
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产品特性
- Molar Ratio: 3.0 moles TRITC per mol of Chicken IgG
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纯化方法
- Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and purification of the IgG fraction by DEAE-Chromatography.
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免疫原
- Purified free Human Secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Mouse anti-Human IgA (Heavy Chain) antibody
ELISA, WB, IHC (fro), IP Monoclonal Mc24-2E11 IgG1 unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Absorption / Emission: 550 nm / 570 nm
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore by adding 1.0 mL of sterile distilled water
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浓度
- 10 mg/mL
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缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins
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储存液
- Without preservative
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注意事项
- If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -28 °C for longer.
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- IgA
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物质类
- Antibody
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背景
- Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM.Synonyms: Human Immunoglobulin A
抗原
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