驴 anti-人 IgM Antibody (PE) - Preadsorbed
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Quick Overview for 驴 anti-人 IgM Antibody (PE) - Preadsorbed (ABIN5608181)
抗原
片段
克隆类型
标记
应用范围
亚型
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适用
- 人
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宿主
- 驴
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Supplier Product No.
- 709-708-031
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Supplier
- Rockland
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原理
- F(ab')2 Human IgM Fc5u Antibody Phycoerythrin Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Bv & Hs Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Human IgM, Human IgM Fc5μ and Human Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Bovine or Horse Serum Proteins, or other human heavy or light chain proteins.
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产品特性
- F(ab')2 Anti-Human IgG (H&L) Biotin Antibody generated in donkey detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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免疫原
- Optional[Immunogen]: Human IgM Fc5mu fragment
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Mouse anti-Human IgM antibody (FITC)
FACS Monoclonal CH2 IgG1 kappa FITC
Mouse anti-Human IgM antibody (APC)FACS Monoclonal CH2 IgG1 kappa APC
Mouse anti-Human IgM antibody (PE)FACS Monoclonal CH2 IgG1 kappa PE
Mouse anti-Human IgM antibody (iFluor™647)GMP Grade FACS Monoclonal M-2E6 IgG1 iFluor™647
Mouse anti-Human IgM antibody (iFluor™700)GMP Grade FACS Monoclonal M-2E6 IgG1 iFluor™700
Mouse anti-Human IgM antibody (mFluor™450)GMP Grade FACS Monoclonal M-2E6 IgG1 mFluor™450
Mouse anti-Human IgM antibody (PE-Cy7)GMP Grade FACS Monoclonal M-2E6 IgG1 PE-Cy7
Goat anti-Human IgM antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human IgM (Heavy Chain) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Human IgM (Heavy Chain) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
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应用备注
- Application Note: F(ab')2 Anti-Human IgM Fc5μ Phycoerythrin Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications. Flow Cytometry Dilution: 1:100 - 1:250 IF Microscopy Dilution: 1:100 - 1:250 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 0.5 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
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有效期
- 6 months
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- IgM
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物质类
- Antibody
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背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
抗原
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