山羊 anti-人 lambda Light Chain (Lambda-IgLC) Antibody (TRITC)
Rockland 709-1011
Lambda-IgLC
FACS, FLISA, FM
Polyclonal
IgG
TRITC
F(ab')2 fragment
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- 抗原 See all Lambda-IgLC products
- Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
- 片段
- F(ab')2 fragment
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- TRITC
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应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 709-1011
- Supplier
- Rockland
- 原理
- F(ab')2 Human (lambda chain) Antibody Rhodamine Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.
- 产品特性
- F(ab')2 Anti-Human (kappa light chain) Antibody generated in goat detects human kappa light chain. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Human (kappa light chain) Antibody is conjugated to Rhodamine.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
- 免疫原
- Optional[Immunogen]: Human lambda light chain
- 亚型
- IgG
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Mouse anti-Human lambda Light Chain (Lambda-IgLC) antibody
Lambda-IgLC FACS, IF Monoclonal IgG1 unconjugated
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- 应用备注
- Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 2.5 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
- 别名
- lambda light chain (Lambda-IgLC 产品)
- 背景
- F(ab')2 Anti-Human (lambda light chain) Antibody generated in goat detects human lambda light chain. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Human (lambda light chain) Antibody is conjugated to rhodamine.
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