山羊 anti-大鼠 IgA, IgG, IgM Antibody (Biotin)
ELISA, IHC, WB
Polyclonal
IgG
Biotin
Quick Overview for 山羊 anti-大鼠 IgA, IgG, IgM Antibody (Biotin) (ABIN5608151)
抗原
IgA, IgG, IgM
克隆类型
多克隆
标记
Biotin
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
亚型
IgG
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适用
- 大鼠
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宿主
- 山羊
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Supplier Product No.
- 612-106-130
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Supplier
- Rockland
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原理
- Rat IgG IgA IgM (H&L) Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgA, Rat IgG and Rat IgM. This reagent is suitable for the detection of all rat immunoglobulin subclasses, isotypes and chain combinations.
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产品特性
- Anti-Rat IgG (H&L) generated in goat detects rat Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG is conjugated to biotin.
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纯化方法
- This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
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Immunogen: Anti-Rat IgG IgA IgM (H&L) was produced by repeated immunization with Rat IgG, IgA and IgM whole molecules in goat.
Immunogen Type: Native Protein
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应用备注
- Application Note: Anti-Rat IgG IgA IgM (H&L) Biotin conjugated antibodies has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:10,000 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:150,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgA, IgG, IgM
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别名
- IgG IgA IgM
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物质类
- Antibody
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背景
- Anti-Rat IgG IgA IgM (H&L) biotin conjugated antibody generated in goat detects specifically rat IgG IgA IgM (H&L). This secondary biotin conjugated antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
抗原
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