兔 anti-小鼠 kappa Light Chain Antibody (HRP)
Rockland 610-4310
WB, ELISA, IHC
Polyclonal
IgG
HRP
-
- 抗原 See all kappa Light Chain products
- kappa Light Chain
-
适用
- 小鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- HRP
-
应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 610-4310
- Supplier
- Rockland
- 原理
- Mouse (kappa chain) Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other mouse heavy or light chain isotypes.
- 产品特性
- Anti-Mouse IgM (mu chain) peroxidase conjugated antibody generated in rabbit detects specifically mouse IgM.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
-
Immunogen: Anti-Mouse kappa light chain was produced by repeated immunization with Mouse kappa light chain in rabbit.
Immunogen Type: Native Protein
- 亚型
- IgG
-
-
- 应用备注
- Application Note: Anti-Mouse kappa light chain antibody generated in rabbit detects specifically Mouse kappa light chain. Anti-Mouse κ Peroxidase Conjugated Antibody has been tested by ELISA and is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:5,000 ELISA Dilution: 1:10,000 - 50,000 Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Thimerosal - 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- kappa Light Chain
- Abstract
- kappa Light Chain 产品
- 背景
- Anti-Mouse κ (kappa chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse kappa light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG kappa is conjugated to peroxidase.
-
You are here:
