山羊 anti-人 kappa Light Chain Antibody
Rockland 609-1110
WB, ELISA, IHC
Polyclonal
IgG
unconjugated
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- 抗原 See all kappa Light Chain products
- kappa Light Chain
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 609-1110
- Supplier
- Rockland
- 原理
- Human (kappa chain) Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. Specificity for kappa chain was confirmed by ELISA minimal cross reactivity against other human heavy or lambda light chain isotypes.
- 产品特性
- Anti-Human IgM (mu heavy chain) generated in goat detects specifically Human IgM mu heavy chain.
- 纯化方法
- Human κ (kappa chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 过滤
- Sterile filtered
- 免疫原
- Optional[Immunogen]: Human kappa light chain
- 亚型
- IgG
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- 应用备注
- Application Note: Anti-Human κ antibody has been tested by ELISA and western blot and is suitable for immunohistochemistry and immunoassays where specificity to the immunoglobulin kappa subunit is desired. Optimal concentrations in immunoassays should be determined by the researcher. Immunohistochemistry Dilution: 1:500 - 1:2,000 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:20,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- kappa Light Chain
- Abstract
- kappa Light Chain 产品
- 背景
- The anti-Human κ (kappa chain) Antibody detects the kappa chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain.
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