山羊 anti-大鼠 IgA Antibody
ELISA, WB, IHC
Polyclonal
IgG
unconjugated
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- 抗原 See all IgA products
- IgA
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Supplier Product No.
- 612-1106
- Supplier
- Rockland
- 原理
- Rat IgA (alpha chain) Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against Rat IgM or Rat IgG. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes.
- 产品特性
- Anti-Rat IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
- 纯化方法
- Anti-Rat IgA was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 过滤
- Sterile filtered
- 免疫原
- Optional[Immunogen]: Rat IgA alpha heavy chain
- 亚型
- IgG
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- 应用备注
- Application Note: Anti-Rat IgA antibody is suitable for Elisa, Western Blots and Immunohistochemistry. Antibody should be optimized by the end user for specific conditions of reactivity. Immunohistochemistry Dilution: 1:500 - 1:3,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- IgA
- Abstract
- IgA 产品
- 物质类
- Antibody
- 背景
- Rat IgA antibodies detects the alpha chain of the rat IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against Rat IgM or Rat IgG. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes. Anti-Rat IgA antibody is ideal for investigators involved in serum component research.
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