山羊 anti-大鼠 IgA Antibody
ELISA, WB, IHC
Polyclonal
IgG
unconjugated
Quick Overview for 山羊 anti-大鼠 IgA Antibody (ABIN5608139)
抗原
IgA
克隆类型
多克隆
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
亚型
IgG
-
-
适用
- 大鼠
-
宿主
- 山羊
-
Supplier Product No.
- 612-1106
-
Supplier
- Rockland
-
原理
- Rat IgA (alpha chain) Antibody
-
交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against Rat IgM or Rat IgG. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes.
-
产品特性
- Anti-Rat IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
-
纯化方法
- Anti-Rat IgA was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
-
过滤
- Sterile filtered
-
免疫原
- Optional[Immunogen]: Rat IgA alpha heavy chain
-
-
-
-
应用备注
- Application Note: Anti-Rat IgA antibody is suitable for Elisa, Western Blots and Immunohistochemistry. Antibody should be optimized by the end user for specific conditions of reactivity. Immunohistochemistry Dilution: 1:500 - 1:3,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
-
有效期
- 12 months
-
-
- IgA
-
物质类
- Antibody
-
背景
- Rat IgA antibodies detects the alpha chain of the rat IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against Rat IgM or Rat IgG. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes. Anti-Rat IgA antibody is ideal for investigators involved in serum component research.
抗原
-
You are here:
