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山羊 anti-人 IgG Antibody

ELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
Rockland
产品编号 ABIN5608134
Supplier Product No.: 709-1110
发货至: 中国
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Our Local Distributor

中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for 山羊 anti-人 IgG Antibody (ABIN5608134)

抗原

IgG

片段

F(ab')2 fragment

克隆类型

  • 3429
  • 166
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  • 1
多克隆

应用范围

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ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

亚型

IgG
  • 适用

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    宿主

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    山羊

    Supplier Product No.

    709-1110

    Supplier

    Rockland

    原理

    F(ab')2 Human (kappa chain) Antibody

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgA and Human Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).  Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.

    产品特性

    F(ab')2 Anti-Human IgA Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

    纯化方法

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

    过滤

    Sterile filtered

    免疫原

    Immunogen: Anti-Human IgG (kappa chain) was produced by repeated immunization with human IgG kappa light chain in goat.

    Immunogen Type: Native Protein

  • 应用备注

    Application Note: Anti-Human IgG (kappa chain) Antibody has been tested by ELISA and western blot and is suitable for immunohistochemistry assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:25,000 Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.0 mg/mL

    缓冲液

    Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

    有效期

    12 months
  • 抗原

    IgG

    物质类

    Antibody

    背景

    Anti-Human IgG (kappa chain) antibody generated in goat detects specifically human IgG (kappa chain). This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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