山羊 anti-雪貂 IgA Antibody (Biotin)
ELISA, WB, IHC
Polyclonal
IgG
Biotin
Quick Overview for 山羊 anti-雪貂 IgA Antibody (Biotin) (ABIN5608124)
抗原
IgA
克隆类型
多克隆
标记
Biotin
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
亚型
IgG
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适用
- 雪貂
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宿主
- 山羊
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Supplier Product No.
- 618-106-006
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Supplier
- Rockland
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原理
- Ferret IgA (alpha chain) Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Ferret IgA and Ferret Serum. No reaction was observed against Ferret IgM or Ferret IgG.
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产品特性
- Anti-Ferret IgG IgA IgM Alkaline Phosphatase Antibody generated in goat detects immunoglobulin G, A, and M from ferret. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain.
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纯化方法
- Anti-FERRET IgA (alpha chain) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Optional[Immunogen]: Ferret IgA alpha heavy chain
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应用备注
- Application Note: Anti-FERRET IgA (alpha chain) (GOAT) Antibody has been assayed against 1.0 μg of Ferret IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 is suggested for this product. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgA
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物质类
- Antibody
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背景
- Anti-Ferret IgA Biotin Antibody generated in goat detects immunoglobulin A from ferret. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA).
抗原
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