山羊 anti-小鼠 IgA, IgG, IgM Antibody (FITC) - Preadsorbed
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北京 101111
Quick Overview for 山羊 anti-小鼠 IgA, IgG, IgM Antibody (FITC) - Preadsorbed (ABIN5608110)
抗原
片段
克隆类型
标记
应用范围
亚型
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适用
- 小鼠
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宿主
- 山羊
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Supplier Product No.
- 710-102-130
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Supplier
- Rockland
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原理
- F(ab')2 Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed
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交叉反应 (详细)
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Human Serum Proteins. This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.
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产品特性
- F(ab')2 Anti-Mouse IgG IgA IgM (H&L) Fluorescein Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM subclasses and chains. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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纯化方法
- This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Optional[Immunogen]: Mouse IgG IgA and IgM whole molecule
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应用备注
- Application Note: F(ab')2 Anti-Mouse IgG IgA IgM (H&L) Fluorescein Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: User Optimized IF Microscopy Dilution: 1:500 - 1:2,500 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgA, IgG, IgM
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别名
- IgG,IgA,IgM
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物质类
- Antibody
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背景
- F(ab')2 Anti-Mouse IgG IgA IgM (H&L) Fluorescein Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM subclasses and chains. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
抗原
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