山羊 anti-大鼠 IgG (F(ab')2 Region) Antibody (HRP) - Preadsorbed
Quick Overview for 山羊 anti-大鼠 IgG (F(ab')2 Region) Antibody (HRP) - Preadsorbed (ABIN5608100)
抗原
片段
克隆类型
标记
应用范围
亚型
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抗原表位
- F(ab')2 Region
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适用
- 大鼠
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宿主
- 山羊
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Supplier Product No.
- 712-1331
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Supplier
- Rockland
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原理
- F(ab')2 Rat IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
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产品特性
- F(ab')2 Anti-Rat IgG F(c) Peroxidase Antibody was generated in goat and detects specifically Rat IgG F(c). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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免疫原
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Immunogen: Anti-Rat IgG F(ab')2 fragment was produced by repeated immunization with Rat IgG F(ab')2 fragment in goat.
Immunogen Type: Native Protein
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Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 488) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG Atto 488
Goat anti-Rat IgG (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Mouse IgG antibody (HRP) - PreadsorbedELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment
Goat anti-Rat IgG antibody (DyLight 488)WB, FLISA, FM Polyclonal IgG DyLight 488
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG Atto 647N
Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rat IgG antibody (Cy3) - PreadsorbedWB, FACS, FLISA, FM, DB Polyclonal IgG Cy3
Goat anti-Rat IgG (Heavy Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
Goat anti-Rat IgG (Heavy Chain) antibody (beta-Gal)ELISA, IHC, WB Polyclonal IgG beta-Gal
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应用备注
- Application Note: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:500 - 1:5,000 Western Blot Dilution: 1:5,000 - 1:200,000 ELISA Dilution: 1:5,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
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浓度
- 0.8 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- F(ab')2 Anti-Rat IgG F(ab')2 Peroxidase Antibody generated in goat detects Rat F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
抗原
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