山羊 anti-人 lambda Light Chain (Lambda-IgLC) Antibody (Alkaline Phosphatase (AP))
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北京 101111
Quick Overview for 山羊 anti-人 lambda Light Chain (Lambda-IgLC) Antibody (Alkaline Phosphatase (AP)) (ABIN5608097)
抗原
片段
克隆类型
标记
应用范围
亚型
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适用
- 人
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宿主
- 山羊
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Supplier Product No.
- 709-1511
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Supplier
- Rockland
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原理
- F(ab')2 Human (lambda chain) Antibody Alkaline Phosphatase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.
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产品特性
- Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Human lambda light chain
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Rabbit anti-Human lambda Light Chain (Lambda-IgLC) (AA 1-216) antibody
Lambda-IgLC WB, IHC (p) Polyclonal unconjugated
Goat anti-Human lambda Light Chain (Lambda-IgLC) antibodyLambda-IgLC WB, IHC, ELISA Polyclonal IgG unconjugated
Goat anti-Human lambda Light Chain (Lambda-IgLC) antibody (HRP)Lambda-IgLC WB, IHC, ELISA Polyclonal IgG HRP
Goat anti-Human lambda Light Chain (Lambda-IgLC) antibody (HRP)Lambda-IgLC WB, IHC, ELISA Polyclonal IgG HRP F(ab')2 fragment
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应用备注
- Application Note: This product has been assayed against 1.0 μg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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- Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
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别名
- lambda light chain
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背景
- Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
抗原
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