山羊 anti-雪貂 IgG Antibody (Alkaline Phosphatase (AP))
Quick Overview for 山羊 anti-雪貂 IgG Antibody (Alkaline Phosphatase (AP)) (ABIN5608092)
抗原
克隆类型
标记
应用范围
亚型
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适用
- 雪貂
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宿主
- 山羊
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Supplier Product No.
- 618-105-012
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Supplier
- Rockland
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原理
- Ferret IgG (gamma chain) Antibody Alkaline Phosphatase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Ferret IgG and Ferret Serum. No reaction was observed against Ferret IgA or Ferret IgM. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Ferret heavy or light chain isotypes.
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产品特性
- Anti-Ferret IgM antibody specifically detects ferret IgM.
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纯化方法
- Anti-Ferret IgG (gamma chain) was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Ferret IgG gamma heavy chain
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Goat anti-Ferret IgG antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Ferret IgG antibody (Biotin)ELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Ferret IgG antibodyELISA, WB Polyclonal IgG unconjugated
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应用备注
- Application Note: Anti-Ferret IgG (gamma chain) antibody is suitable for ELISA, Western Blot and Immunohistochemistry. Antibody should be optimized by end user for reactive conditions. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
抗原
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