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山羊 anti-人 lambda Light Chain (Lambda-IgLC) Antibody

Rockland 709-1111 Lambda-IgLC WB, IHC, ELISA Polyclonal IgG unconjugated F(ab')2 fragment
Rockland
产品编号 ABIN5608051
Supplier Product No.: 709-1111
发货至: 中国
  • 抗原 See all Lambda-IgLC products
    Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
    片段
    F(ab')2 fragment
    适用
    • 31
    • 11
    宿主
    • 20
    • 12
    • 9
    • 1
    山羊
    克隆类型
    • 29
    • 13
    多克隆
    应用范围
    • 27
    • 22
    • 21
    • 20
    • 10
    • 10
    • 9
    • 9
    • 7
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Supplier Product No.
    709-1111
    Supplier
    Rockland
    原理
    F(ab')2 Human (lambda chain) Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).  Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.
    产品特性
    Anti-Human IgG (kappa chain) antibody generated in goat detects specifically human IgG (kappa chain). This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    纯化方法
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
    过滤
    Sterile filtered
    免疫原
    Optional[Immunogen]: Human lambda light chain
    亚型
    IgG
  • 应用备注
    Application Note: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:6,000 Other: User Optimized
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
    有效期
    12 months
  • 抗原
    Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
    别名
    lambda light chain (Lambda-IgLC 产品)
    背景
    Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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