山羊 anti-人 lambda Light Chain (Lambda-IgLC) Antibody
Quick Overview for 山羊 anti-人 lambda Light Chain (Lambda-IgLC) Antibody (ABIN5608051)
抗原
片段
克隆类型
应用范围
亚型
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适用
- 人
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宿主
- 山羊
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Supplier Product No.
- 709-1111
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Supplier
- Rockland
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原理
- F(ab')2 Human (lambda chain) Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.
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产品特性
- Anti-Human IgG (kappa chain) antibody generated in goat detects specifically human IgG (kappa chain). This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Human lambda light chain
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Goat anti-Human lambda Light Chain (Lambda-IgLC) antibody
Lambda-IgLC WB, IHC, ELISA Polyclonal IgG unconjugated
Goat anti-Human lambda Light Chain (Lambda-IgLC) antibody (HRP)Lambda-IgLC WB, IHC, ELISA Polyclonal IgG HRP
Goat anti-Human lambda Light Chain (Lambda-IgLC) antibody (HRP)Lambda-IgLC WB, IHC, ELISA Polyclonal IgG HRP F(ab')2 fragment
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应用备注
- Application Note: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:6,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
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别名
- lambda light chain
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背景
- Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
抗原
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