山羊 anti-人 IgA Antibody
Quick Overview for 山羊 anti-人 IgA Antibody (ABIN5608046)
抗原
片段
克隆类型
应用范围
亚型
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适用
- 人
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宿主
- 山羊
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Supplier Product No.
- 709-1106
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Supplier
- Rockland
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原理
- F(ab')2 Human IgA (alpha chain) Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.
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产品特性
- F(ab')2 Anti-Human IgG IgA IgM (H&L) Texas Red Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Human IgA alpha heavy chain
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Rabbit anti-Human IgA antibody
IHC Monoclonal MSVA-700R IgG Recombinant Antibody unconjugated
Mouse anti-Human IgA (Heavy Chain) antibodyELISA, WB, IHC (fro), IP Monoclonal Mc24-2E11 IgG1 unconjugated
Goat anti-Human IgA antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgA antibody (HRP) - PreadsorbedELISA Polyclonal IgG HRP
Goat anti-Human IgA antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human IgA (Heavy Chain) antibody (Biotin)ELISA, WB, FACS Polyclonal IgG Biotin
Goat anti-Human IgA (Heavy Chain) antibody (FITC)ELISA, FACS Polyclonal IgG FITC
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应用备注
- Application Note: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.3 mg/mL
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缓冲液
- Optional[Buffer]: None, Preservative:None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Secondary Antibody at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IgA
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物质类
- Antibody
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背景
- F(ab')2 Anti-Human IgA Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
抗原
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