山羊 anti-人 IgA, IgG, IgM Antibody (HRP)
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北京 101111
Quick Overview for 山羊 anti-人 IgA, IgG, IgM Antibody (HRP) (ABIN488655)
抗原
克隆类型
标记
应用范围
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适用
- 人
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宿主
- 山羊
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特异性
- Human IgA+IgG+IgM heavy chains and light chains on all Human immunoglobulins.
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预测反应
- human IgA+IgG+IgM heavy chains and light chains on all human immunoglobulins
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产品特性
- Goat anti-human IgA+IgG+IgM is a secondary antibody conjugated to HRP which binds to human immunoglobulins in immunological assays.
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纯化方法
- Affinity purification
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免疫原
- affinity purified human IgA+IgG+IgM
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应用备注
- 1: 10 000 -1: 50 000 (ELISA), (WB), 1: 500 -1: 5000 (IHC)
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说明
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No reactivity to non immunoglobulin human proteins based on immunoelectrophoresis
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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缓冲液
- 10 mM Sodium Phosphate, 0.15 M SodiumChloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.
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储存液
- Kathon CG
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注意事项
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Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. -
储存条件
- 4 °C
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储存方法
- Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- IgA, IgG, IgM
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别名
- IgA+IgG+IgM
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物质类
- Antibody
抗原
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