山羊 anti-Turkey IgG (Heavy & Light Chain) Antibody (Biotin)
ELISA, WB, ICC, DB, IHC (p)
Polyclonal
Biotin
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- Turkey
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Turkey IgG, heavy and light chains.
- 交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in immunoelectrophoresis a precipitin line with pigeon, Duck and Chicken has been observed.
- 产品特性
- The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in turkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
- 纯化方法
- Purified
- 免疫原
- Purified normal IgG isolated from pooled turkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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- 应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline. No preservative added. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
- 缓冲液
- Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline. No preservative added.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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