兔 anti-大鼠 IgA, IgG, IgM (Heavy & Light Chain) Antibody - Preadsorbed
ELISA, WB, DB, IFA
Polyclonal
IgG
unconjugated
产品编号 ABIN458756
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 兔 anti-大鼠 IgA, IgG, IgM (Heavy & Light Chain) Antibody - Preadsorbed (ABIN458756)
抗原
IgA, IgG, IgM
克隆类型
多克隆
应用范围
ELISA, Western Blotting (WB), Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)
亚型
IgG
-
-
抗原表位
- Heavy & Light Chain
-
适用
- 大鼠
-
宿主
- 兔
-
原理
- Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains)
-
特异性
- Purified IgG fraction of polyclonal Rabbit antiSerum to Rat immunoglobulins IgG, IgA, IgM, heavy and light chains.
-
交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. Cross-reactivity of this antiSerum has not been tested in detail.
-
产品特性
- The defined antibody specificity is directed to the major isotypes of the rat immunoglobulin system (classes, subclasses and L chain types) including antibody to common determinants, to class and subclass-specific determinants, and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
-
纯化方法
- Purified IgG fraction from antiserum
-
免疫原
- Highly purified IgG, including all subclasses, and pools of homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
-
-
-
应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
-
缓冲液
- Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
-
-
- IgA, IgG, IgM
-
别名
- IgG + IgA + IgM
-
物质类
- Antibody
抗原
-
You are here:
