绵羊 anti-大鼠 IgG2b (Fc Region) Antibody
ID, IEP
Polyclonal
IgG
unconjugated
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- 抗原 See all IgG2b products
- IgG2b
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抗原表位
- Fc Region
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适用
- 大鼠
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宿主
- 绵羊
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克隆类型
- 多克隆
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应用范围
- Immunodiffusion (ID), Immunoelectrophoresis (IEP)
- 特异性
- The reactivity of the antiserum is directed to the Fc portion of the subclass IgG2b.
- 产品特性
- Total protein and IgG concentration in the antiserum are comparable to those of pooled normal sheep serum. Antibody titre: Precipitin titre not less then 1:64 when tested against pooled normal rat serum in agar block immunodiffu
- 纯化方法
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Delipidated, heat inactivated, lyophilized whole antiserum.
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. - 免疫原
- Pools of purified homogenous IgG2b isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2b in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG2b from serum or plasma.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized a ntiserum by adding 1 mL sterile distilled water.
- 缓冲液
- No foreign proteins added.
- 储存液
- Without preservative
- 注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not ref rozen, and preferably used the same day.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- 抗原
- IgG2b
- Abstract
- IgG2b 产品
- 物质类
- Antibody
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