绵羊 anti-大鼠 IgG1 (Subclass specific) Antibody
IEP, ODD
Polyclonal
IgG
unconjugated
产品编号 ABIN458733
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 绵羊 anti-大鼠 IgG1 (Subclass specific) Antibody (ABIN458733)
抗原
IgG1
克隆类型
多克隆
应用范围
Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD)
亚型
IgG
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抗原表位
- Subclass specific
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适用
- 大鼠
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宿主
- 绵羊
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原理
- Sheep anti Rat IgG1 (subclass specific)
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特异性
- Precipitating polyclonal Sheep antiSerum to Rat IgG1, subclass specific.
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交叉反应 （详细）
- This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail, however in double radial immunodiffusion a reaction with Mouse has been obtained.
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产品特性
- The reactivity of the antiserum is directed to the Fc portion of the subclass IgG1. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG1 from serum or plasma.
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纯化方法
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
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免疫原
- Pools of purified homogenous IgG1 isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- Precipitation assays. In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added as it may interfere with he antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal sheep serum. No foreign
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
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- IgG1
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物质类
- Antibody
抗原
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